Negative control of plasmid DNA replication by iterons. Correlation with initiator binding affinity.

摘要: Initiation of DNA replication and negative control initiation frequency in many bacterial plasmids are mediated by multiple binding sites (iterons) for the replicon-encoded initiator protein. Here we show that a single iteron plasmid P1, when cloned multicopy vector, pUC19, can miniP1 effectively. Using this assay system, several iterons with point mutations were analyzed. The degree was directly correlated affinity protein vitro. also unaffected whether or not wild type flanked strong transcription terminators. We conclude is only activity P1 sequences relevant to control.