P1 plasmid replication. Role of initiator titration in copy number control.
作者： Subrata K. Pal , Rebecca J. Mason , Dhruba K. Chattoraj
DOI: 10.1016/0022-2836(86)90364-5
关键词:
摘要: The copy number control locus incA of unit plasmid P1 maps in a region containing nine 19 base-pair repeats. Previous results from studies vivo and vitro indicated that interacts with the plasmid-encoded RepA protein, which is essential for replication. It has been proposed repeat sequences negatively by sequestering rate-limiting Our lend further support to this hypothesis. Here we show repeats can be deleted completely miniplasmids deletion an approximately eightfold increase number. So, are totally dispensable replication have only regulatory role. incA-deleted plasmids reduced if present trans or reincorporated at two different positions plasmid. This reduction not due lowered expression repA gene presence incA. We one sequence sufficient bind reduce plasmids. When part was deleted, it lost its ability as well influence These strong correlation between capacity protein both vitro, function
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sci-hub.se 参考文章(20)
Ann L. Abeles, Krista M. Snyder, Dhruba K. Chattoraj, P1 plasmid replication: replicon structure. Journal of Molecular Biology. ,vol. 173, pp. 307- 324 ,(1984) , 10.1016/0022-2836(84)90123-2
Jack J. Manis, Bruce C. Kline, Restriction endonuclease mapping and mutagenesis of the F sex factor replication region. Molecular Genetics and Genomics. ,vol. 152, pp. 175- 182 ,(1977) , 10.1007/BF00268815
Stuart Austin, Ann Abeles, Partition of unit-copy miniplasmids to daughter cells: I. P1 and F miniplasmids contain discrete, interchangeable sequences sufficient to promote equipartition Journal of Molecular Biology. ,vol. 169, pp. 353- 372 ,(1983) , 10.1016/S0022-2836(83)80055-2
Y Terawaki, Y Itoh, Copy mutant of mini-Rts1: lowered binding affinity of mutated RepA protein to direct repeats. Journal of Bacteriology. ,vol. 162, pp. 72- 77 ,(1985) , 10.1128/JB.162.1.72-77.1985
L A Rokeach, L Søgaard-Andersen, S Molin, Two functions of the E protein are key elements in the plasmid F replication control system. Journal of Bacteriology. ,vol. 164, pp. 1262- 1270 ,(1985) , 10.1128/JB.164.3.1262-1270.1985
Allan M. Maxam, Walter Gilbert, [57] Sequencing end-labeled DNA with base-specific chemical cleavages Nucleic Acids Part I. ,vol. 65, pp. 499- 560 ,(1980) , 10.1016/S0076-6879(80)65059-9
A L Abeles, P1 plasmid replication. Purification and DNA-binding activity of the replication protein RepA. Journal of Biological Chemistry. ,vol. 261, pp. 3548- 3555 ,(1986) , 10.1016/S0021-9258(17)35681-8
Hiroshi Tsutsui, Asao Fujiyama, Tomoaki Murotsu, Kenichi Matsubara, Role of Nine Repeating Sequences of the Mini-F Genome for Expression of F-Specific Incompatibility Phenotype and Copy Number Control Journal of Bacteriology. ,vol. 155, pp. 337- 344 ,(1983) , 10.1128/JB.155.1.337-344.1983
J. Light, S. Molin, Post-transcriptional control of expression of the repA gene of plasmid R1 mediated by a small RNA molecule. The EMBO Journal. ,vol. 2, pp. 93- 98 ,(1983) , 10.1002/J.1460-2075.1983.TB01387.X
David D. Womble, Padmini Sampathkumar, Alan M. Easton, Verne A. Luckow, Robert H. Rownd, Transcription of the replication control region of the IncFII R-plasmid NR1 in vitro and in vivo. Journal of Molecular Biology. ,vol. 181, pp. 395- 410 ,(1985) , 10.1016/0022-2836(85)90228-1