Control of glucose-fed batch cultivations of E. coli by means of an oxygen stabilized enzyme electrode

作者: Neil Cleland , Sven-Olof Enfors

DOI: 10.1007/BF00498035

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摘要: The design of an autoclavable oxygen stabilized electrode based on immobilized glucose oxidase and catalase, with electrolytic generation oxygen, is presented. electrode's behaviour was investigated in relation to parameters such as ion strength, buffer capacity, DOT the broth enzyme inhibition by Cl−. It used fed-batch cultivations Escherichia coli for control monitoring feeding. Use liquid feeding data from glucose- well pH growth characterization outlined.

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