Determination of Nucleic Acids on the Basis of Enhancement Effect of Resonance Light Scattering of Toluidine Blue
作者： Li-Hua Chen , Yun-Tong Nie , Liu-Zhan Liu , Han-Xi Shen
DOI: 10.1081/AL-120017266
关键词:
摘要: Abstract Based on a detailed study of the interaction toluidine blue (TB) with nucleic acids, novel resonance light scattering (RLS) method was developed for determination acids. The RLS intensity TB at 400 nm greatly enhanced in presence acids pH 3.17. Linear relationships between and concentration were observed range 0.0–2.7 µg cm−3 calf thymus DNA (ctDNA), 0.0–2.2 µg cm−3 fish sperm (fsDNA), 0.0–0.5 µg cm−3 yeast RNA (yRNA), respectively. limits detection (3σ) determined to be 24.0 ng cm−3 ctDNA, 22.7 ng cm−3 fsDNA 5.1 ng cm−3 yRNA. binding constants evaluated 1.1 × 104 dm3 mol−1 1.8 × 104 dm3 mol−1 fsDNA, 1.7 × 104 dm3 mol−1 maximum numbers (n) 2.7, 5.5, 15.0 yRNA, proposed applied chicken blood. Th...
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