Gamma-ray susceptibility of immature and mature hippocampal cultured cells.
作者： Myoung-Sub SONG , Joong-Sun KIM , Miyoung YANG , Sung-Ho KIM , Jong-Choon KIM
DOI: 10.1292/JVMS.09-0553
关键词:
摘要: Ionizing radiation suppresses neurogenesis in the mammalian brain. This vitro study compared detrimental effect of acute gamma-irradiation on immature hippocampal cells with mature cells. Both rat (0.5 day (DIV)) and (14 DIV) were irradiated 0-4 Gy gamma-rays. Cell viability was analyzed by using a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. DNA fragmentation performed extracting intracellular DNA. Morphological features apoptosis characterized 4',6-diamidine-2'-phenylindole, dihydrochloride (DAPI) staining. MTT assay revealed that survival rate declined dose-dependent manner within range irradiation applied, but not changed Intranucleosomal ladder like pattern dose-dependently increased cells, The number apoptotic nuclei significantly applied. Active caspase-3 cleaved poly (ADP-ribose) polymerase (PARP) expressions at 6 hr after 2 exposure markedly higher than control levels. greater radiosensitivity indicates susceptibility such depends their maturation. In addition, may induce caspase-dependent
cabdirect.org
sci-hub.se 参考文章(22)
Glenn T. Gobbel, Mattia Bellinzona, Axel R. Vogt, Nalin Gupta, John R. Fike, Pak H. Chan, Response of Postmitotic Neurons to X-Irradiation: Implications for the Role of DNA Damage in Neuronal Apoptosis The Journal of Neuroscience. ,vol. 18, pp. 147- 155 ,(1998) , 10.1523/JNEUROSCI.18-01-00147.1998
C. Verderio, S. Coco, E. Pravettoni, A. Bacci, M. Matteoli, Synaptogenesis in hippocampal cultures Cellular and Molecular Life Sciences CMLS. ,vol. 55, pp. 1448- 1462 ,(1999) , 10.1007/S000180050384
Muneesh Tewari, Long T Quan, Karen O'Rourke, Serge Desnoyers, Zhi Zeng, David R Beidler, Guy G Poirier, Guy S Salvesen, Vishva M Dixit, Yama/CPP32β, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase Cell. ,vol. 81, pp. 801- 809 ,(1995) , 10.1016/0092-8674(95)90541-3
H Fukuda, A Fukuda, C Zhu, L Korhonen, J Swanpalmer, S Hertzman, M Leist, B Lannering, D Lindholm, T Björk-Eriksson, I Marky, K Blomgren, Irradiation-induced progenitor cell death in the developing brain is resistant to erythropoietin treatment and caspase inhibition. Cell Death & Differentiation. ,vol. 11, pp. 1166- 1178 ,(2004) , 10.1038/SJ.CDD.4401472
Per Hall, Hans-Olov Adami, Dimitrios Trichopoulos, Nancy L Pedersen, Pagona Lagiou, Anders Ekbom, Martin Ingvar, Marie Lundell, Fredrik Granath, Effect of low doses of ionising radiation in infancy on cognitive function in adulthood: Swedish population based cohort study BMJ. ,vol. 328, pp. 19- 0 ,(2004) , 10.1136/BMJ.328.7430.19
Cynthia M. Simbulan-Rosenthal, Dean S. Rosenthal, Sudha Iyer, A. Hamid Boulares, Mark E. Smulson, Transient Poly(ADP-ribosyl)ation of Nuclear Proteins and Role of Poly(ADP-ribose) Polymerase in the Early Stages of Apoptosis Journal of Biological Chemistry. ,vol. 273, pp. 13703- 13712 ,(1998) , 10.1074/JBC.273.22.13703
Carsten Culmsee, Subbarao Bondada, Mark P. Mattson, Hippocampal neurons of mice deficient in DNA-dependent protein kinase exhibit increased vulnerability to DNA damage, oxidative stress and excitotoxicity. Molecular Brain Research. ,vol. 87, pp. 257- 262 ,(2001) , 10.1016/S0169-328X(01)00008-0
John R. Fike, Susanna Rosi, Charles L. Limoli, Neural precursor cells and central nervous system radiation sensitivity. Seminars in Radiation Oncology. ,vol. 19, pp. 122- 132 ,(2009) , 10.1016/J.SEMRADONC.2008.12.003
Katsuyuki Shirai, Toshiyuki Mizui, Yoshiyuki Suzuki, Yasuhiko Kobayashi, Takashi Nakano, Tomoaki Shirao, Differential effects of x-irradiation on immature and mature hippocampal neurons in vitro. Neuroscience Letters. ,vol. 399, pp. 57- 60 ,(2006) , 10.1016/J.NEULET.2006.01.048
Donald W. Nicholson, Ambereen Ali, Nancy A. Thornberry, John P. Vaillancourt, Connie K. Ding, Michel Gallant, Yves Gareau, Patrick R. Griffin, Marc Labelle, Yuri A. Lazebnik, Neil A. Munday, Sayyaparaju M. Raju, Mark E. Smulson, Ting-Ting Yamin, Violeta L. Yu, Douglas K. Miller, Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis Nature. ,vol. 376, pp. 37- 43 ,(1995) , 10.1038/376037A0