Inhibition of key enzymes linked to type 2 diabetes and sodium nitroprusside induced lipid peroxidation in rats' pancreas by phenolic extracts of avocado pear leaves and fruit.

摘要: Persea americana fruit and leaves had been known in folk medicine for their anti-diabetic prowess. Therefore, this study sought to investigate the inhibitory effect of phenolic extract from avocado pear (Persea americana) fruits on some key enzymes linked type 2 diabetes (α-amylase α-glucosidase); sodium nitroprusside (SNP) induced lipid peroxidation rats' pancreas vitro. The extracts were extracted using methanol 1M HCl (1:1 v/v). Thereafter, effects α-glucosidase) determined result revealed that inhibit both α-amylase α-glucosidase activities a dose dependent manner. However, Peel highest activity while leaf as by IC50 value. Furthermore, incubation rat presence 5 mM SNP caused an increase malondialdehyde (MDA) content tissue, however, introduction inhibited MDA produced additive and/or synergistic action major compounds such syringic acid, eugenol, vnillic isoeugenol, guaiacol, kaemferol, catechin, ρ-hydroxybenzoic ferulic apigenin, naringenin, epigallocatechin, epicatechin, lupeol epigallocatechin-3-O-gallate gas chromatography (GC) could have contributed observed medicinal properties plant. inhibition prevention oxidative stress be possible mechanism which they exert properties.