Involvement of the Xenopus laevis Ro60 autoantigen in the alternative interaction of La and CNBP proteins with the 5′UTR of L4 ribosomal protein mRNA

摘要: Abstract In vertebrates the synthesis of ribosomal proteins is co-ordinately regulated at translational level. The 5′-untranslated region (5′UTR) this class mRNAs contains conserved regions that are necessary and sufficient for regulation. Recently, we found two proteins, Xenopus laevis La autoantigen cellular nucleic acid binding protein (CNBP), able to bind in vitro a pyrimidine tract 5′ end downstream region, respectively. These considered common cis-acting elements It was previously observed both these putative trans-acting factors their RNA sequences assisted by protease-sensitive factor(s) dissociates from complex after its formation. Here provide evidence requirement an ancillary factor assisting typical RNA, secondly it may involve recognition motif not clearly characterized previously. We also show Ro60 involved activity CNBP respective sequences. addition, our findings suggest participates process. can multimerise binds 5′UTR as dimer. Both compete interaction with factor, mutually exclusive. Our results analysis mutations 5′UTR, which known disrupt control vivo, model interactions structure co-operate regulating fate mRNAs.