Immunization with recombinantly expressed glycan antigens from Schistosoma mansoni induces glycan-specific antibodies against the parasite

摘要: Schistosomiasis caused by infection with parasitic helminths of Schistosoma spp. is a major global health problem due to inadequate treatment and lack vaccine. The immune response schistosomes includes glycan antigens, which could be valuable diagnostic markers vaccine targets. However, no precedent exists for how design vaccines targeting eukaryotic glycoconjugates. di- tri-saccharide motifs LacdiNAc (GalNAcβ1,4GlcNAc; LDN) fucosylated (GalNAcβ1,4(Fucα1-3)GlcNAc; LDNF) are the basis several important schistosome antigens. They occur in monomeric form or as repeating units (poly-LDNF) part variety different Because chemical synthesis conjugation such antigens exceedingly difficult, we sought develop recombinant expression system parasite glycans. We hypothesized that presentation glycans on cell surface would induce glycan-specific antibodies. generated Chinese hamster ovary (CHO) Lec8 lines expressing poly-LDN (L8-GT) poly-LDNF (L8-GTFT) abundantly their membrane glycoproteins. Sera from mansoni-infected mice were highly cross-reactive cells cell-surface N-glycans. Immunizing L8-GT L8-GTFT induced sustained booster response, antibodies bound S. mansoni lysates recapitulated exquisite specificity anti-parasite particular presentations LDNF antigen. In summary, this promotes successful generation mansoni, it can adapted study role anti-glycan responses many other infections pathologies.