The extracellular space and epileptic activity in the adult brain: Explaining the antiepileptic effects of furosemide and bumetanide

摘要: Treatments that modulate the size of extracellular space (ECS) also block epileptiform activity in adult brain tissue. This includes loop diuretics furosemide and bumetanide, alterations osmolarity ECS. These treatments a variety laboratory seizure models regardless underlying synaptic physiologic mechanisms generating activity. Optical imaging studies on hippocampal slices show blockade by these is concomitant with their activity-driven changes Here we develop analyze hypothesis ECS are necessary for maintenance hypersynchronous In support this an accumulation data from number suggesting bumetanide mediate antiepileptic effects through cell swelling, dependent antagonism glial Na+-K-2Cl cotransporter (NKCC1).