Histone deacetylase inhibitors (HDACIs) induce HIF-1 transcriptional activity.

摘要: 5730 Combined deletions of 1p and 19q are associated with gliomas oligodendroglial lineage. Gliomas these have a significantly better prognosis often response to chemotherapy radiation therapy. By LOH CGH analysis the usually involve whole arms. Because oligodendrogliomas been difficult culture in vitro, there very few accurate cytogenetic studies tumors. Using modifications various stem cell media we able perform short-term recovery metaphases from small number In one oligodendroglioma observed an unbalanced 45,XX,-1,-19,+t(1;19)(q10;p10) karyotype all abnormal metaphases. We developed bacterial artificial chromosome (BAC) FISH probe for proximal 19p12 green combined it 1 alpha satellite (CEP1) orange. applied this mixture metaphase cells interphase nuclei glioma. Fusion CEP1 probes was as well 74% nuclei. then set sections 89 formalin-fixed paraffin-embedded gliomas. The prevalence CEP1/19p12 fusion 72% among 54 oligodendrogliomas, 50% 16 mixed oligoastrocytomas, 10% 19 astrocytomas. 96% 27 13% 30 without 1p/19q deletion had probes. Two five solitary or also evidence CEP1/19q12 fusion. patients enrolled on two NCCTG low-grade glioma trials were included above studies. predict prospectively-collected (Buckner et al. ASCO 2005). currently evaluating whether population patients. summary, our results strongly suggest that t(1;19)(q10;p10) mediates human translocation likely involves centromere both chromosomes is possible more than gene development (Supported part by CA85799, CA108961 grants CA25224 CA114740)