Double-stranded siRNA targeted to the huntingtin gene does not induce DNA methylation
作者： Chang Won Park , Zongyu Chen , Betsy T. Kren , Clifford J. Steer
DOI: 10.1016/J.BBRC.2004.08.096
关键词:
摘要: RNA interference is an evolutionarily conserved mechanism of post-transcriptional gene silencing. Small interfering RNAs (siRNA) 21-23 nucleotides generated from processing double-stranded (dsRNA) by ribonuclease III, Dicer, are widely used for selective sequence-specific silencing in a broad range organisms. In plants, siRNA associated with de novo RNA-directed DNA methylation (RdDM) at the homologous target genomic region. To examine RdDM somatic cells, human glioblastoma cell lines were treated siRNAs to huntingtin responsible Huntington's disease. Methylation CpG dinucleotides plasmid vectors expressing dsRNAs and region was investigated bisulfite-mediated sequencing. Target regions showed no significant change pattern methylation, observed on vectors. These results indicate that not directly linked locus cells.
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