RNA interference-inducing hairpin RNAs in plants act through the viral defence pathway
作者： Adriana F Fusaro , Louisa Matthew , Neil A Smith , Shaun J Curtin , Jasmina Dedic‐Hagan
关键词:
摘要: RNA interference (RNAi) is widely used to silence genes in plants and animals. It operates through the degradation of target mRNA by endonuclease complexes guided approximately 21 nucleotide (nt) short interfering RNAs (siRNAs). A similar process regulates expression some developmental nt microRNAs. Plants have four types Dicer-like (DCL) enzyme, each producing small with different functions. Here, we show that DCL2, DCL3 DCL4 Arabidopsis both replicating viral RNAi-inducing hairpin (hpRNAs) into 22-, 24- siRNAs, respectively, loss DCL2 activities required negate RNAi release plant's repression replication. We also hpRNAs, infection, can engender long-distance silencing signals hpRNA-induced suppressed a virus-derived suppressor protein. These findings indicate hpRNA-mediated defence pathway.
uq.edu.au
elsevier.com
nature.com
core.ac.uk 参考文章(26)
Steve Rozen, Helen Skaletsky, Primer3 on the WWW for general users and for biologist programmers. Methods of Molecular Biology. ,vol. 132, pp. 365- 386 ,(2000) , 10.1385/1-59259-192-2:365
Patrice Crété, Sabrina Leuenberger, Victor A. Iglesias, Vivian Suarez, Hanspeter Schöb, Hauke Holtorf, Sjoerd Van Eeden, Frederick Meins, Graft transmission of induced and spontaneous post-transcriptional silencing of chitinase genes. Plant Journal. ,vol. 28, pp. 493- 501 ,(2001) , 10.1046/J.1365-313X.2001.01171.X
R. A. Jefferson, T. A. Kavanagh, M. W. Bevan, GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants. The EMBO Journal. ,vol. 6, pp. 3901- 3907 ,(1987) , 10.1002/J.1460-2075.1987.TB02730.X
Wonkeun Park, Junjie Li, Rentao Song, Joachim Messing, Xuemei Chen, CARPEL FACTORY, a Dicer Homolog, and HEN1, a Novel Protein, Act in microRNA Metabolism in Arabidopsis thaliana Current Biology. ,vol. 12, pp. 1484- 1495 ,(2002) , 10.1016/S0960-9822(02)01017-5
Patrice Dunoyer, Christophe Himber, Olivier Voinnet, DICER-LIKE 4 is required for RNA interference and produces the 21-nucleotide small interfering RNA component of the plant cell-to-cell silencing signal Nature Genetics. ,vol. 37, pp. 1356- 1360 ,(2005) , 10.1038/NG1675
Olivier Voinnet, Induction and suppression of RNA silencing: insights from viral infections Nature Reviews Genetics. ,vol. 6, pp. 206- 220 ,(2005) , 10.1038/NRG1555
Colin G. N. Turnbull, Jon P. Booker, H. M. Ottoline Leyser, Micrografting techniques for testing long-distance signalling in Arabidopsis Plant Journal. ,vol. 32, pp. 255- 262 ,(2002) , 10.1046/J.1365-313X.2002.01419.X
Neil A. Smith, Surinder P. Singh, Ming-Bo Wang, Peter A. Stoutjesdijk, Allan G. Green, Peter M. Waterhouse, Total silencing by intron-spliced hairpin RNAs Nature. ,vol. 407, pp. 319- 320 ,(2000) , 10.1038/35030305
Peter M. Waterhouse, Ming-Bo Wang, Tony Lough, Gene silencing as an adaptive defence against viruses Nature. ,vol. 411, pp. 834- 842 ,(2001) , 10.1038/35081168
E.Jean Finnegan, Rogerio Margis, Peter M Waterhouse, Posttranscriptional Gene Silencing Is Not Compromised in the Arabidopsis CARPEL FACTORY (DICER-LIKE1) Mutant, a Homolog of Dicer-1 from Drosophila Current Biology. ,vol. 13, pp. 236- 240 ,(2003) , 10.1016/S0960-9822(03)00010-1