In situ sequencing for RNA analysis in preserved tissue and cells

作者: Rongqin Ke , Marco Mignardi , Alexandra Pacureanu , Jessica Svedlund , Johan Botling

DOI: 10.1038/NMETH.2563

关键词:

摘要: … that included four degenerated nucleotides (NNNN) that upon sequencing should generate … Detection probes that contained uracils were first treated with UNG treating buffer (1× phi29 …

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