Possible indirect detection of rHuEPO administration in human urine by high-performance liquid chromatography tandem mass spectrometry.

摘要: The present study is based on the assumption that changes in an ADMA-DDAH-NOS (ADMA-asymmetrical dimethylarginine; DDAH-dimethyl-arginine dimethylaminohydrolase; NOS-nitric oxide synthase) system could be employed as indirect markers for recombinant human erythropoietin (rHuEPO) administration doping control. We assessed a predictive value of four proposed new rHuEPO abuse. Preliminary data showed concentrations ADMA, symmetrical dimethylarginine (SDMA), citrulline and arginine urine were increased after single intravenous injection (2000 U day(-1), Epocrine, St-Petersburg, Russia). variations SDMA, levels before was performed with two healthy male volunteers. Urine samples collected urinary ADMA SDMA determined at 10.0-40 microg mL(-1) 0.5-10 mL(-1). A dose caused increase up to 40-270 mL(-1), 40-240 10-60 12-140 respectively. These preliminary results indicated approach used pre-screening order decrease number low probability abuse and, thus, save costs workload.