Urea, the most abundant component in urine, cross-reacts with a commercial 8-OH-dG ELISA kit and contributes to overestimation of urinary 8-OH-dG

作者: Ming-Fen Song , Yun-Shan Li , Yuko Ootsuyama , Hiroshi Kasai , Kazuaki Kawai

DOI: 10.1016/J.FREERADBIOMED.2009.02.017

关键词:

摘要: Urinary 8-OH-dG is commonly analyzed as a marker of oxidative stress. For its analysis, ELISA and HPLC methods are generally used, although discrepancies in the data obtained by …

参考文章(22)
K C Cheng, D S Cahill, H Kasai, S Nishimura, L A Loeb, 8-Hydroxyguanine, an abundant form of oxidative DNA damage, causes G----T and A----C substitutions. Journal of Biological Chemistry. ,vol. 267, pp. 166- 172 ,(1992) , 10.1016/S0021-9258(18)48474-8
S. Toyokuni, K. Uchida, T. Osawa, H. Hiai, Y. Hattori, A. Yoshida, Y. Nishiyama, T. Tanaka, H. Ochi, Quantitative immunohistochemical determination of 8-hydroxy-2'-deoxyguanosine by a monoclonal antibody N45.1: its application to ferric nitrilotriacetate-induced renal carcinogenesis model Laboratory Investigation. ,vol. 76, pp. 365- 374 ,(1997)
Marcus S. Cooke, Mark D. Evans, Karl E. Herbert, Joseph Lunec, Urinary 8-oxo-2'-deoxyguanosine--source, significance and supplements. web science. ,vol. 32, pp. 381- 397 ,(2000) , 10.1080/10715760000300391
M. K. Shigenaga, C. J. Gimeno, B. N. Ames, Urinary 8-hydroxy-2'-deoxyguanosine as a biological marker of in vivo oxidative DNA damage. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 9697- 9701 ,(1989) , 10.1073/PNAS.86.24.9697
Svetlana A. Appolonova, Marina A. Dikunets, Grigory M. Rodchenkov, Possible indirect detection of rHuEPO administration in human urine by high-performance liquid chromatography tandem mass spectrometry. European Journal of Mass Spectrometry. ,vol. 14, pp. 201- 209 ,(2008) , 10.1255/EJMS.922
J.-L Ravanat, B Duretz, A Guiller, T Douki, J Cadet, Isotope dilution high-performance liquid chromatography–electrospray tandem mass spectrometry assay for the measurement of 8-oxo-7,8-dihydro-2′-deoxyguanosine in biological samples Journal of Chromatography B: Biomedical Sciences and Applications. ,vol. 715, pp. 349- 356 ,(1998) , 10.1016/S0378-4347(98)00259-X
P PATEL, R BEVAN, N MISTRY, J LUNEC, Evidence of oligonucleotides containing 8-hydroxy-2'-deoxyguanosine in human urine. Free Radical Biology and Medicine. ,vol. 42, pp. 552- 558 ,(2007) , 10.1016/J.FREERADBIOMED.2006.11.025
Hiroshi Kasai, Kazuaki Kawai, Yun-shan Li, Analysis of 8-OH-dG and 8-OH-Gua as Biomarkers of Oxidative Stress Genes and Environment. ,vol. 30, pp. 33- 40 ,(2008) , 10.3123/JEMSGE.30.33
Hiroshi Kasai, Hisako Hayami, Ziro Yamaizumi, Hazime Saito, Susumu Nishimura, Detection and identification of mutagens and carcinogens as their adducts with guanosine derivatives Nucleic Acids Research. ,vol. 12, pp. 2127- 2136 ,(1984) , 10.1093/NAR/12.4.2127