Protein Purification by Selective Adsorption on Immobilized Pyridinium Ligands
作者： That T. Ngo
DOI: 10.1007/978-1-4757-9519-6_18
关键词:
摘要: Avid AL is an affinity gel with synthetic low molecular weight ligands capable of selectively purifying IgG from the serum all animals tested. By packing in a radial flow column, high rate can be applied and 12 g highly purified obtained within 5 hours directly serum. Immunoglobulins bound to eluted 0.05 M sodium acetate at pH 3 or 7.4 neutral buffer containing “electron-rich” compounds. The appears suitable under varieties storage depyrogenation conditions.
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