Characterization of an R-binding site mediating the R-induced activation of the Epstein-Barr virus BMLF1 promoter.

摘要: In cells latently infected with Epstein-Barr virus, the switch from latency to productive infection is linked expression of two virus transcription factors called EB1 and R. R an enhancer factor, R-responsive element (RRE) has been identified in BMLF1 promoter. this study, we have used bidirectional deletion mutagenesis delineate RRE (RRE-M) a 44-bp sequence. We also show that expressed recombinant vaccinia protects RRE-M against digestion by DNase I. Using mobility shift assays dimethyl sulfate interferences, characterized contact points between vitro-translated DNA. binds vitro one site simultaneously contacting sequences within site, which are separated 8 bp: 5'-catGTCCCtctatcatGGCGCagac-3'. Site-directed sequence completely impaired binding rendered promoter nonresponsive The results suggest R-inducible composed single R-binding RRE-M.