Promoter Elements of the Mouse Acetylcholinesterase Gene TRANSCRIPTIONAL REGULATION DURING MUSCLE DIFFERENTIATION

摘要: Abstract The increase in acetylcholinesterase expression during muscle differentiation from myoblasts to myotubes was shown previously reflect primarily a greater stability of the messenger RNA (mRNA). Here, we investigate regulation gene early determination phenotype. (i) We employ myogenic transcription factors transform non-muscle cells into order assess role this regulation. (ii) analyze Ache promoter region by deletion analysis, point mutagenesis, and gel mobility shift assays. do not accelerate spite presence E-boxes at −335 base pairs start first intron, they are able trigger stabilization mRNA when constitutively expressed 10T1/2 fibroblasts. A GC-rich (at −105 −59 transcription) containing overlapping binding sites for Sp1 Egr-1 is essential activity. Mutation dramatically reduces activity while mutation has little effect. compete an decreases gene.