Sulfenyl Halides as Modifying Reagents for Polypeptides and Proteins. Quantitative Evaluation of Tryptophan and Cysteine Residues in Proteins
作者： Enrico Boccu , Francesco M. Veronese , Angelo Fontana , Carlo A. Benassi
DOI: 10.1111/J.1432-1033.1970.TB00917.X
关键词:
摘要: The determination of tryptophan and cysteine content in proteins by means the selective reaction sulfenyl halides with these amino acids has been carefully investigated. By 2- or 4-nitrophenylsulfenyl chloride aqueous acetic formic acid, nitrophenyl chromophore is covalently bound to residues protein, giving a thioether mixed disulfide respectively. determined spectrophotometrically at 450 nm from 4-nitrothiophenol released disulfides after exposure chloride-labelled protein 0.1 N NaOH (at this wavelength corresponding does not absorb). Tryptophan estimated sulfenylated spectrophotometric determinations 365 328 chromophores (ɛM 4000 11700 respectively), following removal yellow label residues, dissolving modified NaOH. procedure shown be accurate for both wide variety proteins.
sci-hub.se 参考文章(16)
JR Brown, BS Hartley, Location of disulphide bridges by diagonal paper electrophoresis. The disulphide bridges of bovine chymotrypsinogen A. Biochemical Journal. ,vol. 101, pp. 214- 228 ,(1966) , 10.1042/BJ1010214
Christian B. Anfinsen, Edgar Haber, Studies on the reduction and re-formation of protein disulfide bonds. Journal of Biological Chemistry. ,vol. 236, pp. 1361- 1363 ,(1961) , 10.1016/S0021-9258(18)64177-8
DJ WINZOR, JM CREETH, Physicochemical studies on ovalbumin. 3. The sulphydryl and disulphide contents of ovalbumin and an iodine-modified derivative. Biochemical Journal. ,vol. 83, pp. 559- 566 ,(1962) , 10.1042/BJ0830559
W. Duane Brown, Marino Martinez, Harold S. Olcott, Sulfhydryl Groups of Tuna Myoglobins and Hemoglobins, Whale Hemoglobin, Tobacco Mosaic Virus, and Ovalbumin Journal of Biological Chemistry. ,vol. 236, pp. 92- 95 ,(1961) , 10.1016/S0021-9258(18)64435-7
Robert E. Canfield, Anne K. Liu, THE DISULFIDE BONDS OF EGG WHITE LYSOZYME (MURAMIDASE). Journal of Biological Chemistry. ,vol. 240, pp. 1997- 2002 ,(1965) , 10.1016/S0021-9258(18)97416-8
T.E. Barman, D.E. Koshland, A colorimetric procedure for the quantitative determination of tryptophan residues in proteins. Journal of Biological Chemistry. ,vol. 242, pp. 5771- 5776 ,(1967) , 10.1016/S0021-9258(18)99367-1
Robert E. Canfield, The Amino Acid Sequence of Egg White Lysozyme Journal of Biological Chemistry. ,vol. 238, pp. 2698- 2707 ,(1963) , 10.1016/S0021-9258(18)67888-3
Arthur M. Crestfield, Stanford Moore, William H. Stein, The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins. Journal of Biological Chemistry. ,vol. 238, pp. 622- 627 ,(1963) , 10.1016/S0021-9258(18)81308-4
B. S. HARTLEY, J. R. BROWN, DOROTHY L. KAUFFMAN, L. B. SMILLIE, Evolutionary Similarities between Pancreatic Proteolytic Enzymes Nature. ,vol. 207, pp. 1157- 1159 ,(1965) , 10.1038/2071157A0
B. S. HARTLEY, AMINO-ACID SEQUENCE OF BOVINE CHYMOTRYPSINOGEN-A. Nature. ,vol. 201, pp. 1284- 1287 ,(1964) , 10.1038/2011284A0