On the localisation and transport of α-amylase during germination and early seedling growth of Hordeum vulgare

摘要: A procedure for the isolation of a germination-specific α-amylase isoenzyme from Hordeum vulgare (cv. Nordal) is described. The pure was used to obtain monospecific anti-α-amylase antibody. antibody subsequently localise in sections germinating barley seeds Nordal). Immunohistochemical determination pattern enzyme movement during germination and early seedling growth revealed that at commencement protein moiety moves away entire face scutellum intermediate crushed cell layer. As continues, begins be synthesised aleurone layer transported this into endosperm.