Alterations in DNA methylation corresponding with lung inflammation and as a biomarker for disease development after MWCNT exposure.

摘要: Use of multi-walled carbon nanotubes (MWCNT) is growing which increases occupational exposures to these materials. Their toxic potential makes it important have an in-depth understanding the inflammation and disease that develops due exposure. Epigenetics one area interest has been quickly developing assess processes its ability change gene expression thus lung environment after In this study, promoter methylation inflammatory genes (IFN-γ TNF-α) was measured MWCNT exposure using pyrosequencing assay found correlate with initial cytokine production. addition, a involved in tissue fibrosis (Thy-1) also altered way matched collagen deposition. addition epigenetics better understand processes, used as biomarker disease. global determined ascertain whether alter at site if those alterations coincide development. Then, levels were blood could be more easily accessible tissue. Using LuUminometric Methylation Assay (LUMA) 5-Methylcytosine (5-mC) Quantification assay, we lead DNA hypomethylation blood, coincided This study provides data showing gene-specific correspond response coincides MWCNT-induced development, suggesting both