作者: Leslie Ogorzaly , Adeline Tissier , Isabelle Bertrand , Armand Maul , Christophe Gantzer
DOI: 10.1016/J.WATRES.2008.12.011
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摘要: Recent studies have shown the increasing interest of F-specific RNA phage genotyping to identify major sources faecal contamination in waters. This study, conducted a river located an urbanized watershed with recognized anthropogenic influences, was aimed at evaluating relevance direct by real-time RT-PCR. One hundred percent positive results were obtained 5 mL aliquot water (n=31). Phage distribution modified after cultivation, since ratio two most abundant genogroups (II and I) reached 1.51 log(10) RT-PCR-based method versus 0.30 cultivation (n=8). For first time, correlations between concentrations genogroup II, bacterial indicators human adenoviruses observed, which may indicate pollution. No correlation II I has been revealed. The concentration only correlated turbidity, suggesting animal pollution coming from upstream rainfall events. Among microbiological parameters studied, II/genogroup shows variations occurring