Hydrolysis of liquid pineapple waste by invertase immobilized in pva-alginate matrix.

摘要: Abstract A thorough investigation of sucrose hydrolysis by invertase immobilized in recently developed PVA–alginate beads was performed. The bead is distinguished the treatment with sodium sulfate solution. performance matrix terms leakage, reusability, ideal temperature and pH kinetic parameters Km Vmax investigated. Results revealed that for are 50 °C 4 respectively. apparent each relatively lower than free enzyme value 4.7 × 10−5 mM 2.849 × 10−5 mM 12PVA–5BA 12PVA–7BA, An increase also observed 2281.02 U mg−1 reusability its storage period were investigated found to be 14 cycles 60 days, Finally, best (12PVA–5BA) used hydrolyze liquid pineapple waste. production yield glucose about 745% 3 h