Endogenous Levels of Five Fatty Acid Metabolites in Exhaled Breath Condensate to Monitor Asthma by High-Performance Liquid Chromatography: Electrospray Tandem Mass Spectrometry
作者： M.L. Nording , Jun Yang , C.M. Hegedus , A. Bhushan , N.J. Kenyon
DOI: 10.1109/JSEN.2009.2035736
关键词:
摘要: Abstract-Airway inflammation characterizing asthma and other airway diseases may be monitored through biomarker analysis of exhaled breath condensate (EBC). In an attempt to discover novel EBC biomarkers, a high performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method was used analyze from ten control non-asthmatics one asthmatic individual for five fatty acid metabolites: 9,12,13-trihydroxyoctadecenoic (9,12,13-TriHOME), 9,10,13-TriHOME, 12,13-dihydroxyoctadecenoic (12,13-DiHOME), 12-hydroxyeicosatetraenoic (12-HETE), 12(13)-epoxyoctadecenoic (12(13)-EpOME). The shown sensitive, with on-column limit quatitation (LOQ) in the pg range (corresponding pM concentrations EBC), linear over several orders magnitude each analyte calibrated range. Analysis spiked metabolites within 81%-119% only few exceptions. Endogenous levels exhibited intraand inter-assay precision 10%-22%, 12%-36%, respectively. healthy subjects contained average between 15 180 12-HETE present above LOQ at concentration 240 pM. Exposure subject allergen led increased 9,12,13-TriHOME, 12,13-DiHOME, 12(13)-EpOME when compared collected prior exposure (range =40-510 pM). 12,13-DiHOME significantly (Student's t-test, p < 0.05). conclusion, we have developed new HPLC-ESI-MS/MS EBC, which are potential biomarkers monitoring diagnosis.
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