Developmental changes in the cellular distribution of glutathione and glutathione S-transferases in the murine nervous system.

摘要: The distribution of glutathione (GSH) and S-transferases (GSTs) in the adult rat brain is cell-type specific, but their cellular developing central nervous system unknown. In present study, GSH mouse was visualized by mercury orange histochemistry class-specific GSTs were localized immunohistochemistry at ages E13 to PN30. Both neuronal glial progenitor cells stain uniformly positive for E13, Spinal anterior horn neurons become GSH-negative E17, which time glia other CNS regions are still GSH-positive. By PN5, most have lost staining surrounded GSH-rich neuropil, ependyma, vasculature. Olfactory mitral granule cells, cerebellar dorsal root ganglion (DR G) retain consistently high levels throughout development into adulthood. Immunoreactivity α-class GSTantisera not observed until PN10, when very weak becomes apparent pia, choroid plexus spinal cord. μ-GST astrocytes (but oligodendrocytes), PN10. π-GST immunoreactivity all embryonic system. Postnatally, it found oligodendrocytes astrocytes) cord as well plexus. satellite DRG immunoreactive α-,μ-, antisera points examined. developmental changes suggest that enzymatic conjugation antioxidant activities may also be cell specific during development.