A comparison of different methods of β-galactosidase immobilization
作者： A Bodalo , E Gomez , JL Gomez , J Bastida , MF Maximo
DOI: 10.1016/0032-9592(91)85025-J
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摘要: Abstract β-Galactosidase was immobilized in a range of supports showing suitable physico-chemical characteristics for use fluidized bed reactors. Uncoated porous glass, alginate and κ-carrageenan beads chromosorb-W were used as carriers. The intrinsic kinetic constants (V max K M ) coupling parameters the immobilization calculated. highest protein percentages activity yields obtained when β-galactosidase attached through its amine groups to aldehyde-glass. final choice derivative reactors should be based not only on enzymatic shown by derivatives but also hydrodynamic behaviour supports.
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