ABCB1 is involved in vitamin D intestinal efflux

摘要: Recently, a new pathway for cholesterol elimination has been discovered: the transintestinal excretion (TICE). This phenomenon involves ABCB1 (ATP-binding cassette B1). Vitamin D3 (cholecalciferol) shown to be absorbed by enterocytes via transporters. It may thus excreted pathways similar those of cholesterol. As vitamin D is essential calcium and phosphate homeostasis, immune system vascular risk prevention, this situation should considered patients whose TICE would activated. The aim study was assess contribution intestinal efflux (cholecalciferol its metabolite 25-hydroxyvitamin (25(OH)D). Cholecalciferol 25(OH) cells transfected either stably or transiently with gene measured. 25(OH)D status in abcb1-deficient mice compared that wild mice. Their postprandial plasma cholecalciferol response then assessed following gavage. Besides, intestine ability excrete estimated using Ussing chambers situ perfusion experiments. Finally, 39 healthy adult men were genotyped whole-genome microarrays. association between SNPs genes involved lipid metabolism analyzed partial least squares regression (PLS regression). data collected showed overexpressing had better capacity control (+167% +150% 25(OH)D; p<0.05). These results confirmed (p<0.05). In vivo, Abcb1-deficient displayed fasting concentrations 30% higher than type also twice analyses are still ongoing. humans, significant (p = 3.94 x 10-7) PLS model, which comprised 29 10 (including 3 ABCB1), associated 73% interindividual variability concentration. suggest an apical newly- trans-epithelial exists, transporter likely homeostasis.