A microbial biosensor for hydrogen sulfide monitoring based on potentiometry
作者： Ehsan Ebrahimi , Fatemeh Yazdian , Ghasem Amoabediny , Mohammad Reza Shariati , Behdokht Janfada
DOI: 10.1016/J.PROCBIO.2014.05.003
关键词:
摘要: Abstract In this study, a microbial biosensor for hydrogen sulfide (H 2 S) detection based on Thiobacillus thioparus immobilized in gelatin matrix was developed. The T. via either surface adsorption the or entrapment matrix. bacterial and concentration support were then varied order to optimize sensor response time limit both methods. optimization conducted by statistical analysis of measured with various polymer concentrations. According our experiments immobilization methods, optimized conditions method found be 1% an optical density 82. For method, 0.6% 88 selected as optimal conditions. A model developed extent methods immobilization. maximum change potential solution 23.16 mV 34.34 mV absorption method. times 160 s 105 s, respectively. is more advantageous development gas due its shorter time.
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