Heparan sulphate proteoglycan expression in human primary liver tumours

摘要: Heparan sulphate proteoglycans (HSPGs) play important biological roles in cell–matrix adhesion processes and are essential regulators of growth factor actions (e.g., as co-receptor for hepatocyte factor). Since liver carcinogenesis, interactions between cells, the matrix, factors a major role, aim this study was to investigate whether distribution pattern HSPGs is altered human primary tumours. Twenty-two tumours five normal biopsies were studied, using specific monoclonal antibodies against syndecans-1, -2, -3, -4; glypican; perlecan; heparan chains. Cholangiocarcinomas well hepatocellular carcinomas showed an immunoreactivity different comparison with parenchyma, probably reflecting regulatory HSPGs. Intracellular positivity integral membrane syndecan-1 especially syndecan-4 constant finding most tumours, suggesting increased synthesis or internalization these Syndecan-3 perlecan expression found expected pattern. The strong reactivity syndecan-3 tumoral stromal vessels might suggest role angiogenesis. In addition, exerts its known reservoir function also stroma © 1998 John Wiley & Sons, Ltd.