作者: Alicia Orea , Purificación Pajuelo , José M Romero , Antonio J Márquez
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摘要: We have studied the effect of nitrogen supply on growth as well nitrate assimilation in Lotus japonicus plants. High concentrations (higher than 20 mM) were toxic for L japonicus, inhibiting growth, with optimum concentration ranging between 4 and 8 mM. The maximum levels reductase (NR) nitrite (NiR) activity accumulation was obtained plants grown simultaneously plus ammonium. could also grow ammonium sole source, without a supplement organic acids like succinate. Low external induced higher roots, while inhibited roots favoured shoots growth. NR NiR activities always An increase did not alter significantly plant partitioning among shoots, being predominantly stems leaves. kinetics induction by showed different pattern leaves, this independent short-term. In contrast, same dependent concentration. all cases Significant protein but no detected NH + -grown