Characterization of feline T cell receptor gamma (TCRG) variable region genes for the molecular diagnosis of feline intestinal T cell lymphoma.

摘要: Abstract A diagnosis of intestinal lymphoma is currently made on the basis clinical and morphologic criteria. This can prove problematic for many reasons that include inadequate sample size, coexistence inflammation, inability to assess architectural integrity all tissue compartments in biopsy specimens obtained endoscopically. The detection a clonal population cells lymphoproliferative lesion represents an important criterion neoplasia, but this has not been assessed feline lymphoma. T cell receptor gamma ( TCRG ) gene rearrangement analysis using polymerase chain reaction (PCR) methodology be used detect clonality populations. assay depends assessment junctional diversity results from V (variable) J (joining) segments. Feline transcripts normal small intestine spleen were rapid amplification cDNA ends (5′RACE) method. Limited segments was observed. high degree sequence homology exploited develop PCR test V–J hence determination populations cats. Molecular applied lymphoplasmacytic inflammatory bowel disease (IBD) (9 cats), transmural mucosal (28 cats). Clonal junction detected 22 28 lymphomas, oligoclonality 3 lymphomas. contrasted with polyclonal tissues (3 cats) IBD It proposed adjunctive tool cat.