COLON CARCINOMA K-ras 2 ONCOGENE OF A FAMILIAL POLYPOSIS COLI PATIENT
作者： Michiko Miyaki , Takeo Iwama , Chieko Sato , Yasuhito Yuasa , Masayoshi Namba
DOI: 10.20772/CANCERSCI1985.77.9_901
关键词:
摘要: The DNA of a colon carcinoma-derived cell line (KMS-4) and that skin fibroblasts from familial polyposis coli patient were transfected into NIH3T3 cells in order to detect oncogenes associated with the disease. No transformation was observed normal fibroblast DNA, while KMS-4 able transform cells. Through hybridization known oncogene probes, transforming gene found be human activated c-K-ras 2 oncogene. Sequence analysis molecularly cloned showed single nucleotide transition G T at 12th codon. This results substitution cysteine for glycine this position. On using labeled synthetic oligonucleotides mutation codon 12, we carcinoma suggests activation could induction.
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