Characterization of human cytomegalovirus UL84 early gene and identification of its putative protein product.

摘要: The DNA sequence and transcription pattern of human cytomegalovirus early gene UL84 were analyzed. This was mapped within a 2.6-kb PstI fragment located between 0.534 0.545 map unit the large unique segment genome, which is adjacent to pp65 pp71 genes. A 2.0-kb mRNA transcribed from this region in same leftward direction as mRNAs message first detected at 2.5 h postinfection reached maximal level 72 96 postinfection. nucleotide sequences genomic cDNA derived determined. resulting data revealed polyadenylation signal (AATAAA) 14 nucleotides upstream poly(A) tail 1,761-bp open reading frame capable encoding 65-kDa polypeptide. potential leucine zipper found N-terminal half peptide molecule amino acids 114 135. In addition, different periodic repeat with every eighth position 325 373. transcriptional initiation site determined by primer extension analysis. putative TATA box (TATTTAA) 24 bp cap several inverted repeats further box. To test whether encodes virus-specific protein, overexpressed Escherichia coli fusion protein used generate antibodies rabbits. molecular size 65 kDa infected-cell extracts harvested 6 postinfection, but not purified virions, using immunoblot Both nuclear cytoplasmic fluorescences late stages virus infection. From results obtained, we postulate that may be stable, virus-specific, nonstructural forming homo- or heterodimeric molecule.