Retrospective Proteomic Screening of 100 Breast Cancer Tissues

摘要: The present investigation has been conducted on one hundred tissue fragments of breast cancer, collected and immediately cryopreserved following the surgical resection. specimens were selected from patients with invasive ductal carcinoma breast, most frequent potentially aggressive type mammary objective to increase knowledge cancer molecular markers useful for clinical applications. proteomic screening; by 2D-IPG mass spectrometry; allowed us identify two main classes protein clusters: proteins expressed ubiquitously at high levels in all patients; sporadically among same patients. Within group ubiquitous proteins, glycolytic enzymes anti-apoptotic activity predominant. Among sporadic ones, involved cell motility, chaperones detoxification appeared prevalent. data study indicates that primary tumor growth is reasonably supported concurrent events: inhibition apoptosis stimulation cellular proliferation, increased expression multiple functions. second phase evolution can be prematurely scheduled occasional presence motility defenses oxidative stress. We suggest this approach large-scale proteomics currently a valid tool offers opportunity evaluate assay recurrence individual their isoforms short forms, proposed as prognostic indicators susceptibility metastasis operated breast.