Synthesis pattern of matrix metalloproteinases (MMPs) and inhibitors (TIMPs) in human explant organ cultures after treatment with latanoprost and dexamethasone.

摘要: Purpose: To determine changes in production of matrix metalloproteinases (MMPs) and tissue inhibitors (TIMPs) the ciliary body, trabecular meshwork retinal pigment epithelium induced by both prostaglandins corticosteroids. Methods: Explant organ cultures were removed a scleral incision 3 mm posterior to limbus. Retinal was grown confluence. Organ cell treated with latanoprost and/or dexamethasone for 72 h. The activity MMP- 2, -3 -9 assessed using zymography. synthesis pattern MMPs TIMP-1 -2 identified immunostaining. Results: Treatment explant 10 μg/ml mean upregulation MMP-2 36%, MMP-3 112% MMP-9 156% as seen Dexamethasone 500 nm reduced amounts secreted 13%, 69%. not detectable media corticosteroid-treated cultures. addition fLg/ml dexamethasone-treated increased 14%, 43% 49%. Using immunohistochemistry we found staining antibodies against MMP-2, -3, within only lesser degree meshwork. Latanoprost treatment caused an increase 29% (p < 0.0001), 98% 0.0001) 108% 0.0001). 32% 33% 83% Almost no change Neither nor significant 0.93) secretion TIMPs. In non-treated (RPE) cells MMP detected MMP-2. RPE culture did respond either their secretion. Conclusion We profound mild induction through but or cells. Corticosteroids, on other hand, downregulated expression tissues. This inhibiting effect corticosteroids reversed latanoprost.