Enantiomer selective acylation of racemic alcohols by lipases in continuous-flow bioreactors

摘要: Abstract Continuous-flow mode enantiomer selective acetylations of racemic 1-phenylethanol, 1-cyclohexylethanol, and 1-phenylpropan-2-ol ( rac - 1a – c , respectively) with vinyl acetate were performed in small stainless steel packed-bed reactors filled different commercial lipase preparations. In several lipase-filled columns, highly E  > 100) kinetic resolutions these alcohols achieved. most cases, comparison the continuous-flow batch (shake flask) biotransformations indicated similar selectivities ) but higher productivities (specific reaction rate: r corresponding reaction. The effect temperature (0–60 °C) pressure (1–120 bar) on acetylation was investigated an immobilized Candida antarctica B (CaLB) reactor. Pressure had no significant . Expectedly, a monotonous increase specific rate observed within this range. Most surprisingly, selectivity maximum  ∼ 25, at 20 °C) minimum  ∼ 7, 50 °C). reactions CaLB-filled columns successfully applied for preparative scale