Mimicking of Chondrocyte Microenvironment Using In Situ Forming Dendritic Polyglycerol Sulfate-Based Synthetic Polyanionic Hydrogels.
作者： Pradip Dey , Tobias Schneider , Leonardo Chiappisi , Michael Gradzielski , Gundula Schulze-Tanzil
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摘要: A stable polymeric network that mimics the highly polyanionic extracellular cartilage matrix still remains a great challenge. The main aim of this study is to present synthesis dendritic polyglycerol sulfate (dPGS)-based in situ forming hydrogels using strain promoted azide-alkyne cycloaddition reactions. real time rheological has been used characterize hydrogel properties. viability encapsulated human chondrocytes different are monitored live-dead staining. Furthermore, type I and II collagen gene have analyzed. Hydrogels with elastic moduli ranging from 1 5 kPa prepared by varying dPGS amount. chondrocyte found be higher than pure PEG alginate-based after 21 d. cell engineered can explained fact interact proteins responsible for growth proliferation.
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