Gene expression analysis using a highly sensitive DNA microarray for colorectal cancer screening.

摘要: BACKGROUND/AIM Half of all patients with small, right-sided, non-metastatic colorectal cancer (CRC) have negative results for the fecal occult blood test (FOBT). In present study, usefulness CRC screening a highly sensitive DNA microarray was evaluated in comparison that by FOBT using samples. MATERIALS AND METHODS A total 53 and 61 healthy controls were divided into "training" "validation sets". For gene profiling, RNA extracted from 0.5 g feces hybridized to chip. RESULTS The expressions 43 genes significantly higher than (p<0.05). training set, sensitivity specificity chip assay six 85.4% 85.2%, respectively. On other hand, validation 85.2% 85.7%, sensitivities those cases early-CRC, tumor invading up muscularis propria (i.e. surface tumor) subgroups. particular, early-CRC subgroups (p=0.023 0.019, respectively.). CONCLUSION Gene profiling more effective at detecting tumor, early-stage CRC.