Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning
作者： Thomas Tiller , Eric Meffre , Sergey Yurasov , Makoto Tsuiji , Michel C. Nussenzweig
DOI: 10.1016/J.JIM.2007.09.017
关键词:
摘要: … cloning strategy. In summary, our method to clone and express human monoclonal antibodies … requires only small cell numbers and the recombinant antibodies allow direct conclusions …
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