SIMPLIFIED DETERMINATION OF BLOOD ADENOSINE TRIPHOSPHATE USING THE FIREFLY SYSTEM.

摘要: A simplified method is described for the determination of red cell ATP using firefly lantern extract method. Variables investigated include effect time reading, dilution and effective range Excellent recoveries were obtained. Optimal extraction from cells was achieved with a hypotonic buffer at pH 9.2. The could be used acid-citrate-dextrose, heparin or EDTA as an anticoagulant. found to highly specific when nucleotides in normal human blood investigated; only adenosine diphosphate guanosine triphosphate gave slight readings, neither which would significantly affect determinations blood. Normal values 5.45 µmoles ATP/Gm. hemoglobin 1.83 µmoles/ml. heparinized samples. This believed more rapid, reproducible accurate than any previously determination.