Comparative analysis of MBD-seq and MeDIP-seq and estimation of gene expression changes in a rodent model of schizophrenia
作者： Jennifer L. Neary , Stephanie M. Perez , Kara Peterson , Daniel J. Lodge , Melanie A. Carless
DOI: 10.1016/J.YGENO.2017.03.004
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摘要: Abstract We conducted a comparative study of multiplexed affinity enrichment sequence methodologies (MBD-seq and MeDIP-seq) in rodent model schizophrenia, induced by utero methylazoxymethanol acetate (MAM) exposure. also examined related gene expression changes using pooled sample approach. MBD-seq MeDIP-seq identified 769 1771 differentially methylated regions (DMRs) between F2 offspring MAM-exposed rats saline control rats, respectively. The assays showed good concordance, with ~ 56% MBD-seq-detected DMRs being or proximal to DMRs. There was no significant overlap expressed genes, suggesting that DNA methylation regulatory effects may act upon more distal are too subtle detect our Methylation ontology analyses biological processes important schizophrenia pathophysiology, including neuron differentiation, prepulse inhibition, amphetamine response, glutamatergic synaptic transmission regulation, reinforcing the utility MAM for research.
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