Changes in gene expression in rat thymocytes identified by cDNA array support the occurrence of oxidative stress in early magnesium deficiency.

摘要: Magnesium deficiency in experimental animals leads to inflammation, exacerbated immune stress response and a decrease of specific response. It also results significant increase free radical species subsequent tissue injury. An accelerated thymus involution was observed Mg-deficient rats relation enhanced apoptosis susceptibility oxidative stress. To examine the stress-inducing effects low Mg status on thymocytes, cDNA arrays were used evaluate changes gene expression weaning submitted short duration (2 days). Several genes exhibited their caused by before any perceptible modification cell integrity functions. The up-regulated included cytochrome c oxidase, glutathione transferase, CuZn superoxide dismutase, associated with (HSP70 HSP84) involved DNA synthesis repair (GADD45). down-regulated Na/P cotransporter 1. These findings are consistent altered growth, modifications ion fluxes described during deficiency. observation induction protection cells from provides additional evidence role pathobiology this