Molecular Detection of Bioluminescent Dinoflagellates in Surface Waters of the Patagonian Shelf during Early Austral Summer 2008

摘要: We investigated the distribution of bioluminescent dinoflagellates in Patagonian Shelf region using “universal” PCR primers for dinoflagellate luciferase gene. Luciferase gene sequences and single cell tests, conjunction with taxonomic identification by microscopy, allowed us to identify quantify dinoflagellates. compared these data coincidental discrete optical measurements stimulable bioluminescence intensity. Molecular detection showed that were widespread across majority region. Their presence was comparatively underestimated measurements, whose magnitude affected interspecific differences intensity other organisms. microscopy complex hydrography area played an important role determining composition populations. Dinoflagellates absent south Falkland Islands where cold, nutrient-rich, well-mixed waters Falklands Current favoured diatoms instead. Diverse populations present warmer, more stratified as it warmed northwards. Here, population could be related distinct water masses. Our results provide new insight into prevalence demonstrate a molecular approach natural is promising tool ecological studies