Temperature influences β-carotene production in recombinant Saccharomyces cerevisiae expressing carotenogenic genes from Phaffia rhodozyma.

摘要: Red yeast Phaffia rhodozyma is a prominent microorganism able to synthesize carotenoid. Here, three carotenogenic cDNAs of P. CGMCC 2.1557, crtE, crtYB and crtI, were cloned introduced into Saccharomyces cerevisiae INVSc1. The recombinant Sc-EYBI cells could 258.8 ± 43.8 μg g−1 dry cell weight (DCW) β-carotene when growing at 20 °C, about 59-fold higher than in those 30 °C. Additional expression the catalytic domain 3-hydroxy-3-methylglutaryl-coenzyme A reductase from S. (Sc-EYBIH) increased level 528.8 13.3 DCW as 27-fold although grew faster °C Consistent with much transcription crt genes cHMG1 gene was little Meanwhile, accumulation promoted growth. These results reveal influence temperature on biosynthesis may be helpful for improving production cerevisiae.