Improved measurement of LINE-1 sequence methylation for cancer detection.

摘要: Abstract Background Methylation of long interspersed nuclear element-1 (LINE-1) sequences varies among normal cells and it is often decreased in cancer genomes white blood (WBC) patients. Current measurement techniques genome-wide level are inadequate because LINE-1 methylation distinctive at each locus. Here, we improved the detection by combining information pattern level. Methods Combined bisulfite restriction analysis (COBRA) LINE-1, COBRA was used to test cell lines, two oral rinse cohorts, WBC from separated into 4 products depending on statuses 2 CpG dinucleotides, as follows: unmethylated CpGs (uCuC), partial (mCuC), 1 methylated (mC), (uC). Results The association between mCuC uCuC directly correlated (r = 0.4895, p = 0.0009) but inversely (r = − 0.8979, p = 0.0002). Oral AUC values were 0.763 0.926 levels 0.707 0.621, respectively. uCuC, not overall level, differentiated (p = 0.0082 p = 0.4830, respectively). Conclusion represents hypomethylation hypermethylation cells. This improves cancer.