Modulation of cardiac myocyte phenotype in vitro by the composition and orientation of the extracellular matrix.

摘要: Cellular phenotype is the result of a dynamic interaction between cell's intrinsic genetic program and morphogenetic signals that serve to modulate extent which expressed. In present study we have examined how information might be stored in extracellular matrix (ECM) communicated neonatal heart cell (NHC) by cardiac alpha 1 beta integrin molecule. A thin film type I collagen (T1C) was prepared with defined orientation. This achieved applying T1C peripheral edge 100 mm culture dish. The then drawn across surface dish continuous stroke sterile scraper allowed polymerize. When NHCs were cultured on this substrate, they spread, as population, along common axis parallel gel lattice expressed an vivo-like phenotype. Individual displayed elongated, rod-like shape disclosed arrays myofibrils. These phenotypic characteristics maintained for at least 4 weeks primary culture. evolution tissue-like organizational pattern dependent upon specific interactions collagen-based mediated complex. conclusion supported variety experimental results. Altering tertiary structure or blocking domains either chain inhibited expression organization. Neither cell-to-cell contact contractile function necessary induce formation shape. However, beating activity assembly well-differentiated myofibrillar apparatus. data suggest complex serves detect transduce within surrounding matrix.