Anaerobic aspartate metabolism and the formation of alanine in molluscan cardiac muscle: A13C Nmr study

摘要: Carbon nuclear magnetic resonance (13C-NMR) spectroscopic techniques were used to investigate the metabolism of (13C-3>)-aspartate and (13C-4>)-aspartate by in vitro preparations ventricle whelk Busycon contrarium. Under anoxic conditions, there was substantial conversion C-1,4 succinate with no detectable enrichment other compounds. When substrate, C-2,3 succinate. However, C-2 or C-3 positions alanine. This finding suggests that direct transfer carbon from aspartate alanine is minimal under conditions ventricle. Thus, malic enzyme may not be functional decarboxylating direction these conditions.