Transcriptional and posttranscriptional activation of urokinase plasminogen activator gene expression in metastatic tumor cells.

摘要: Urokinase plasminogen activator (uPA) is a serine protease which has frequently been implicated in the process of tumor cell invasion and metastasis. The degree expression mode(s) regulation uPA gene metastatic compared with nonmetastatic cells have not yet addressed. We cloned sequenced full-length rat complementary DNA utilized Northern blot analysis to report that expressed at levels 3.5- 70-fold higher lines than derived from two independent mammary adenocarcinomas. Nuclear run-on assays RNA half-life estimations indicated MAT 13762 adenocarcinoma 3.5-fold derivative (J-clone), due combined increase transcription cytoplasmic stability. By contrast, (and enzyme) were elevated by up clones dimethylbenz(a)anthracene-induced (DMBA-8) predominantly posttranscriptional mechanisms. Moreover, treatment DMBA-8 protein synthesis inhibitors led an nuclear levels, without altering rate transcription. These results suggest addition transcription, events localized nucleus cytoplasm are key determinants activation