Effects on T-cell maturation and proliferation induced by lactational transfer of cyclosporine to nursing pups.

摘要: Background. Pregnancy after allotransplantations is becoming a more common occurrence, and the immunosuppressant of choice cyclosporine (CsA) for these patients. Consequently, effect CsA on prenatal postnatal immune development function in infant an increasingly important clinical issue. The purpose this study was to evaluate potential problems maternal exposure neonatal T-cell maturation proliferation lactational transfer animal model. Methods. administered daily (subcutaneous) 20 days during lactation, beginning day parturition using two dose levels (15 25 mg/kg body weight/day) conjunction with saline controls. Results. Considerable amounts were passed newborn rats blood equal that mothers 25-mg/kg/day 55% 15 dose. There significant reduction thymus/body-weight ratio thymus cellularity pups born dosed at or mg/kg/day CsA. from CsA-exposed showed almost complete loss medullary region no apparent change thymic cortex. CsA-treated their dose) had increase percentage CD4 + CD8 thymocytes decrease percentages , CD3hi, receptor (TCR)hi thymocyte phenotype subsets CD4/CD8 ratios. Thymocyte proliferative responses concanavalin A interleukin-2 also significantly decreased mother pup both doses In contrast splenocyte responses, 15- 25-mg/kg doses. All alterations due back control 30 postweaning cessation. Conclusions. This clearly demonstrates via can cause inhibition lymphoproliferative responsiveness mitogen activation. Although level human transplant patients normally much lower, data indicate increased risk opportunistic infections altered components babies exposed long-term