Suppression of dextran sodium sulfate-induced colitis in mice by zerumbone, a subtropical ginger sesquiterpene, and nimesulide: separately and in combination

摘要: Ulcerative colitis (UC) and Crohn's disease are inflammatory disorders of unknown cause difficult to treat, though some synthetic chemicals, including ligands for peroxisome proliferator-activated receptors (PPARs), anticipated be useful drugs. In contrast, few food phytochemicals have been reported suppress in animal models. The present study was undertaken explore the suppressive efficacy zerumbone (ZER), a sesquiterpenoid rhizome Zingiber zerumbet Smith that is used as condiment Southeast Asian countries known potent suppressant cyclooxygenase (COX)-2 inducible nitric oxide synthase expression cell culture systems. Acute induced by exposing female ICR mice 5% DSS drinking water 1 week. One week prior administration, experimental were fed ZER alone, nimesulide (NIM, selective COX-2 inhibitor) or both combination (1000 ppm each) total 2 weeks. Inflammatory biomarkers, i.e. interleukin (IL)-1alpha IL-1beta, tumor necrosis factor (TNF)-alpha, prostaglandin (PG)E(2) PGF(2alpha) colonic mucosa quantified an enzyme-linked immunosorbent assay conjunction with histological alterations. Oral feeding significantly lowered levels IL-1beta [inhibitory rate (IR)=34%], TNF-alpha (IR=29%), PGE(2) (IR=73%) suppressed DSS-induced colitis, whereas NIM changes without affecting biomarkers. However, their treatment most effective suppressing these Our results suggest novel mitigating UC use agents, different modes actions, may anti-inflammatory strategy.